Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Fixable: Fixable via Lysine. Google Scholar. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. All authors have consented to the submission of this manuscript for publication. The site is secure. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. 4K, b M.W. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). FOIA Drug Deliv Syst. An official website of the United States government. SWC designed and coordinated the research. (2003). Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Pavlov, G.M. 8600 Rockville Pike ; Fran, H.P. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Materiaux 1-5. Crini, G. (2005). At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Epub 2022 Feb 2. Drug Deliv. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. 2011;16(2):518. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Expert Rev Med Devices. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). European Polymer Journal, 37, 619-622. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. (2008). Global hydrodynamic analysis of the molecular flexibility of galactomannans. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Bohdanecky, Miloslav (1996). This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Rotureau, E.; Dellacherie, E.; Durand, A. Gums and stabilisers for the Food Industry, 5, 447-450. Before TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. National Library of Medicine TEWL measurements were performed before and 30min after microporation. government site. 2012;101(8):28619. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. ; van Nostrum, C.F. (2007). Colloid and Polymer Science, 286, 1223-1231. Chem. 2000;3(9):31826. (2012). The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Int J Pharm Investig. ; Picout, D.R. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Drug Deliv. Sung-Wook Choi. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. 2015;23(3):2239. Intrinsic viscosity of bovine serum albumin conformers. Curley SA. Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Progress in Polymer Science, 30, 38-70. In addition, the permeation rate was higher in pig skin than in human skin. 43-1, 115-147. (2013). official website and that any information you provide is encrypted These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Carbohydrate Polymers, 39, 377-380. On single point determination of intrinsic viscosity. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Chen Rong Huei; Min Lang Tsai (1998). Keywords: Federal government websites often end in .gov or .mil. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). (2007). Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Cite this article. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Carbohydrate Polymers, 38, 267-271. Progr. After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Carbohydrate Polymers 95 360-365. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Arond L.H. Table 2 compares the properties of nanoparticles coated with one or other polymer. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Granath, K. A. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Advanced Drug Delivery Reviews, 60, 1650-1662. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. The mechanism of the dextran-induced red blood cell Aggregation. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Journal of Physical Chemistry, 58(11), 953-957. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. ; Aberle, T; Burchard, W. (2000). Polysaccharides-based nanoparticles as drug delivery systems. Polysaccharide hydrogels for modified release formulations. Microneedles and their applications. Format). Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. GYA primarily conducted research. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Article J. Braz. Transdermal drug delivery: overcoming the skins barrier function. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Macromolecules, 33, 5730-5739. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected.
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